科研队伍

孔建强,男,博士,研究员,博士生导师,课题组长。主要从事天然药物的生物合成及合成生物学研究。发表文章80余篇,申请专利十余项(授权5项)。《药学学报》,《Acta Pharmaceutica Sinica B》青年编委,《生物工程学报》编委。《Acta Pharmaceutica Sinica B》, 《ACS Synth Biol》,《Microbial cell Fact》,《Bioresource Technology》,《BMC Plant Biology》,《Biochimie》,《Journal of agricultural and food chemistry》,《Phytochemistry》,《药学学报》和《生物工程学报》等三十余种中外期刊的审稿专家。

1,天然产物生物合成
挖掘参与活性天然产物生物合成相关的途径酶基因,并对其进行功能表征;通过计算机模拟以及定向进化等手段对关键途径酶进行突变,获得活性显著提升,具有预设催化特性的生物催化剂,用于天然产物的酶法合成。
2,合成生物学技术制备天然产物
在不同底盘细胞中重构天然产物生物合成代谢途径及调控网络,获得能制备天然产物的细胞工厂,用于天然产物的生物合成。

1, Xue-Ning Wang, Li-Li Hong, and Jian-Qiang Kong*, Diacerein as a promising acyl donor in biosynthetic acetyl-coa and glycosyl esters mediated by a multifunctional maltose O‑acetyltransferase from Escherichia coli, J. Agric. Food Chem, 2021, 69, 6623−6635
2, Li-Li Hong and Jian-Qiang Kong*, Altering the regioselectivity of cytochrome P450 BM3 variant M13 towards genistein through protein engineering and variation of reaction conditions, ACS Omega, 2020, 5, 49, 32059-32066
3, Yan-Li Xu and Jian-Qiang Kong*, OcUGT1-catalyzing glycodiversification of steroids through glucosylation and transglucosylation actions. Molecules 2020, 25, 475.
4, Qing-Wei Chen, Ting-Gong, Pei-Cheng Zhang, Jian-Qiang Kong*, Seven new 1-oxygenated cholestane glycosides from Ornithogalum saundersiae, Journal of Asian Natural Products Research, 2020, 22(3):201-206.
5, Yu-Jia Sun, Jiu-Ming He and Jian-Qiang Kong*, Characterization of two flavonol synthases with iron-independent flavanone 3-hydroxylase activity from Ornithogalum caudatum Jacq, BMC Plant Biology, 2019, 19:195.
6, Qing-Wei Chen, Xu Zhang, Ting Gong, Wan Gao, Shuai Yuan, Pei-Cheng Zhang*, Jian-Qiang Kong*, Structure and bioactivity of cholestane glycosides from the bulbs of Ornithogalum saundersiae Baker. Phytochemistry, 2019, 164: 206-214.
7, Ming Liu, Jian-Qing Kong*, The enzymatic biosynthesis of acylated steroidal glycosides and their cytotoxic activity. Acta Pharmaceutica Sinica B, 2018, 8(6):981-994.
8, Shuai Yuan, Sen Yin, Ming Liu, Jian-Qiang Kong*, Isolation and characterization of a multifunctional flavonoid glycosyltransferase from Ornithogalum caudatum with glycosidase activity. Scientific Reports, 2018, 8, 5886.
9, Shuai Yuan, Sen Yin, Ming Liu, Jiu-Ming He, Jian-Qiang Kong*, OcUGT1-catalyzed glycosylation of testosterone with alternative donor substrates, Process Biochemistry, 2018,73:82-85.
10, Shuai Yuan, Yan Yang, Jian-Qiang Kong*, Biosynthesis of 7,8-dihydroxyflavone glycosides via OcUGT1-catalyzed glycosylation and transglycosylation, Journal of Asian Natural Products Research, 2018, 20(7):662-674.
11, Xin Liu, Jian-Qiang Kong*, Steroids hydroxylation catalyzed by the monooxygenase mutant 139-3 from Bacillus megaterium BM3, Acta Pharmaceutica Sinica B, 2017, 7(4):510–516.

1.孔建强,尹森,一种来源于虎眼万年青的尿苷-5’-二磷酸木糖差向异构酶,其核苷酸序列及应用,ZL201610147497.9,2020年3月31日授权
2.孔建强,尹森,一种来源于虎眼万年青的尿苷-5’-二磷酸半乳糖差向异构酶,其核苷酸序列及应用,ZL201610147498.3,2020年3月31日授权
3.朱平,王伟,孔建强,戴均贵,程克棣,王丽娜,郑晓东,生产紫穗槐-4,11-二烯和青蒿酸的方法,2012,中国,ZL2007101879228

Baidu
map